Enhancing isobutyric acid production from engineered Acidithiobacillus ferrooxidans cells via media optimization
نویسندگان
چکیده
منابع مشابه
Cytochromes c of Acidithiobacillus ferrooxidans.
The chemolithoautotrophic Gram-negative bacterium Acidithiobacillus ferrooxidans is versatile and can grow on a number of electron donors and acceptors. In the A. ferrooxidans ATCC 23270 genome, computer analysis identified 11 genes encoding putative cytochromes c. At least eight putative cytochromes c were differentiated on gels in ATCC 33020 cells grown on ferrous iron or sulfur. All these cy...
متن کاملPeriplasmic Proteins of the Extremophile Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans is a chemolithoautotrophic acidophile capable of obtaining energy by oxidizing ferrous iron or sulfur compounds such as metal sulfides. Some of the proteins involved in these oxidations have been described as forming part of the periplasm of this extremophile. The detailed study of the periplasmic components constitutes an important area to understand the physiolo...
متن کاملReconstitution of iron oxidase from sulfur-grown Acidithiobacillus ferrooxidans.
The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.
متن کاملAssembly mechanism of [Fe2S2] cluster in ferredoxin from Acidithiobacillus ferrooxidans.
Ferredoxin is a typical iron-sulfur protein that is ubiquitous in biological redox systems. This study investigates the in vitro assembly of a [Fe2S2] cluster in the ferredoxin from Acidithiobacillus ferrooxidans in the presence of three scaffold proteins: IscA, IscS, and IscU. The spectra and MALDI-TOF MS results for the reconstituted ferredoxin confirm that the iron-sulfur cluster was correct...
متن کاملPhylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans.
Polyphasic genotypic analysis of 25 Acidithiobacillus ferrooxidans strains isolated from ores and ore concentrates collected in different regions of the world showed considerable strain heterogeneity. Restriction patterns of the chromosomal DNA of these strains obtained by PFGE were specific for each strain. According to the degree of DNA relatedness, 17 of the 23 strains studied were divided i...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2015
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.25837